Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets

doi: 10.1073/pnas.1619216114

Figure Lengend Snippet: The s.c. modular islet transplants with HUVEC restored normoglycemia. (A) Shown are average nonfasting blood glucose levels for diabetic mice implanted with free islets (n = 6) or islets in modules with (+HUVEC) (n = 6) or without (−HUVEC; collagen only; n = 5) HUVEC or with adMSC and HUVEC (+adMSC) (n = 6). While all free-islet recipients remained hyperglycemic for the entire experiment, islet modules with HUVEC were able to lower glucose levels to normal in all but one animal. Individual glucose values are shown in Fig. S2. Islet modules without HUVEC or with adMSC and HUVEC had some therapeutic effect on the average glucose levels; however, most of these animals remained hyperglycemic. The dotted line represents the threshold for normoglycemia (11.1 mM). (B) Kaplan–Meier plot showing when animals became normoglycemic. Not shown are animals that became normoglycemic but reverted to hyperglycemia within the 21-d observation period. Mice implanted with islet modules with HUVEC reversed diabetes significantly faster than the free-islet group (P = 0.0043, log-rank, Mantel-cox test) or the group with islet modules without HUVEC (P = 0.0196, log-rank, Mantel–Cox test). Numbers in parentheses indicate the fraction of animals that are shown in this plot. (C) IPGTT glucose profile at day 14. Animals were fasted for 4 h before IPGTT was performed. A repeated-measures ANOVA with a Games–Howell post hoc test revealed a significant difference between the profiles of the free-islet group and the nondiabetic control and the groups with islets embedded in modules with or without HUVEC. (D) AUC analysis confirmed that mice in the free-islet group were intolerant of the glucose challenge and that there was a significant difference between the free-islet group and both groups receiving islets embedded in modules with (n = 6) or without (n = 5) HUVEC. There was no significant difference among the islet module groups. *P < 0.05, **P < 0.01.

Article Snippet: In most cases, HUVEC (2.0 × 10 6 cells/mL collagen) (P3-6; Lonza) were seeded dynamically onto the surface of the modules for 60 min in an appropriate coculture medium.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets

doi: 10.1073/pnas.1619216114

Figure Lengend Snippet: Nonfasting blood glucose readings for each animal were taken at days 7, 14, and 21. (A) There were significant differences in the average nonfasting blood glucose levels in animals transplanted with free islets and those implanted with islet modules with HUVEC at each time point. One or two module recipients were normoglycemic at day 7, but only the islet (+HUVEC) group had the majority of animals becoming normoglycemic by day 14 and sustaining normoglycemia (11.1 mM, represented by the dashed line) to day 21). *P < 0.05, **P < 0.01. (B) The percentage of animals that were normoglycemic at days 7, 14, and 21. While two animals in the islets in collagen gel group (islet modules without HUVEC) were normoglycemic at days 7 and 14, these animals became hyperglycemic before day 21; a third animal became normoglycemic at day 20 and remained so until day 21. Animals transplanted with free islets remained hyperglycemic for the entire 21 d.

Article Snippet: In most cases, HUVEC (2.0 × 10 6 cells/mL collagen) (P3-6; Lonza) were seeded dynamically onto the surface of the modules for 60 min in an appropriate coculture medium.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets

doi: 10.1073/pnas.1619216114

Figure Lengend Snippet: Cell compositions of different transplant groups in the study

Article Snippet: In most cases, HUVEC (2.0 × 10 6 cells/mL collagen) (P3-6; Lonza) were seeded dynamically onto the surface of the modules for 60 min in an appropriate coculture medium.

Techniques:

Journal: iScience

Article Title: Implantation of engineered human microvasculature to study human infectious diseases in mouse models

doi: 10.1016/j.isci.2023.106286

Figure Lengend Snippet:

Article Snippet: Rat tail type I collagen for propagation of HUVECs , Becton Dickinson , #11563550.

Techniques: Plasmid Preparation, Construct, Modification, Software